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             «“√ “√°“√·æ∑¬
                                                                     1. Thin Layer Chromatography: TLC

                                                                     2. Gas Chromatography: GC
                      Safety §«“¡ª≈Õ¥¿—¬ ¬“®“° ¡ÿπ‰æ√  Efficacy ª√– ‘∑∏‘º≈  HPLC 3. High Performance Liquid Chromatography:




                                                                     4. Electrophoretic Method
                                   ∑’Ë¥’
                                                                     5. Spectrophotometry

                                                                Chromatographic fingerprint analysis
                             Quality §ÿ≥¿“æ
                                                                      “¡“√∂·∫àßÕÕ°‰¥â‡ªìπ 2 ·∫∫§◊Õ °“√«‘‡§√“–À凙‘ß

             √Ÿª∑’Ë 1 ≈—°…≥– concept ¢Õ߬“®“° ¡ÿπ‰æ√∑’Ë¥’       §ÿ≥¿“æ (qualitative method) ·≈–°“√«‘‡§√“–À凙‘ߪ√‘¡“≥
                                                                (quantitative method)
             À≈—°‡°≥±å«‘∏’°“√∑’Ë¥’„π°“√º≈‘μ (Good Manufacturing      1. Qualitative method of chromatographic fin-

             Practice À√◊Õ GMP) ¬“®“° ¡ÿπ‰æ√ ¡’°“√μ√«® Õ∫ ¡ÿπ   gerprint
             ‰æ√«—μ∂ÿ¥‘∫∑’Ë„™â«à“‡ªìπ™π‘¥∑’Ë∂Ÿ°μâÕß ¡’ “√ ”§—≠„πª√‘¡“≥∑’Ë  ¡’®ÿ¥ª√– ß§å„π°“√¥Ÿ√Ÿª·∫∫Õߧåª√–°Õ∫‚¥¬√«¡

             μâÕß°“√ ‡¡◊ËÕºà“π°√–∫«π°“√º≈‘μ®π·≈⫇ √Á® °ÁμâÕß¡’°“√  (general characteristics) ‡æ◊ËÕ‡ª√’¬∫‡∑’¬∫§«“¡‡À¡◊Õπ
             μ√«® Õ∫«à“¡’ “√ ”§—≠‡ªìπ‰ªμ“¡¡“μ√∞“π∑’Ë°”Àπ¥       (sameness) ·≈–§«“¡μà“ß (differences) ¢Õß«—μ∂ÿ¥‘∫·≈–
             ªí®®ÿ∫—πÕÿμ “À°√√¡º≈‘μ¿—≥±å®“° ¡ÿπ‰æ√¡’°“√¢¬“¬     ¬“®“° ¡ÿπ‰æ√°—∫ “√¡“μ√∞“π‡æ◊ËÕ°“√æ‘ Ÿ®πå‡Õ°≈—°…≥å

             μ—«Õ¬à“ß√«¥‡√Á« ‚¥¬¡’‚√ßß“πÕÿμ “À°√√¡¥—ß°≈à“«ª√–¡“≥  (identification) πÕ°®“°π’È “¡“√∂𔉪À“§«“¡ ¡Ë”‡ ¡Õ
                 2
             1,117  ·Ààß (¢âÕ¡Ÿ≈ ≥ ¡’π“§¡ 2554) ‚¥¬¡’ —¥ à«π¢Õß‚√ßß“π  ¢ÕßÕߧåª√–°Õ∫ (consistency) ¿“¬„π°“√º≈‘μ√ÿàπ‡¥’¬«°—π
                                                  3
             „πμà“ß®—ßÀ«—¥¡“°°«à“„π°√ÿ߇∑未√–¡“≥ 2 ‡∑à“ ·μà¡’‡æ’¬ß  ·≈–√–À«à“ß√ÿàπ∑’Ë°“√º≈‘μ ·≈–¬—ß𔉪„™â„π°“√μ√«® Õ∫
             42 ·Àà߇∑à“π—Èπ∑’ˉ¥â√—∫°“√√—∫√Õß°“√§«∫§ÿ¡§ÿ≥¿“æ°“√º≈‘μ  À“§«“¡§ßμ—« (stability) ‰¥â «‘∏’°“√π’È¡’¢âÕ¥’§◊Õ  “¡“√∂
             μ“¡¡“μ√∞“π “°≈ (Good Manufacturing Practice: GMP)  §«∫§ÿ¡§ÿ≥¿“æ¢Õß«—μ∂ÿ¥‘∫·≈–¬“®“° ¡ÿπ‰æ√‰¥â·¡â«à“®–¬—ß

             ®“° ”π—°ß“π§≥–°√√¡°“√Õ“À“√·≈–¬“ °√–∑√«ß “∏“√≥ ÿ¢   ‰¡à¡’¢âÕ°”Àπ¥ “√¡“μ√∞“π∑’Ë®–π”¡“„™â„π°“√‡ª√’¬∫‡∑’¬∫
                                 4
             (¢âÕ¡Ÿ≈ ≥ ∏—𫓧¡ 2553)                            ¢≥–∑’Ë¢âÕ‡ ’¬¢Õß«‘∏’°“√π’ȧ◊Õ ‡°≥±å∑’Ë„™â„π°“√ª√–‡¡‘πº≈
                 πÕ°®“°π’ÈÕߧ尓√Õπ“¡—¬‚≈° (World Health Organi-  °“√«‘‡§√“–Àå·≈–°“√°”Àπ¥§à“¡“μ√∞“π¬—߉¡à¡’§«“¡™—¥‡®π

             zation: WHO) ‰¥â°”À𥫑∏’°“√§«∫§ÿ¡§ÿ≥¿“æ¢Õß«—μ∂ÿ¥‘∫  ¡’§«“¡¬ÿà߬“° ´—∫´âÕπ ·≈–·μ°μà“ß°—π‰ª„π·μà≈–ª√–‡∑»
             ∑’ˇªìπæ◊™ ¡ÿπ‰æ√∑’Ëπ”¡“∑”‡ªì𬓷≈–º≈‘μ¿—≥±å®“° ¡ÿπ   à«π„À≠à®–¢÷Èπ°—∫™π‘¥¢Õ߇§√◊ËÕß¡◊Õ·≈– software ∑’Ë„™â
             ‰æ√‚¥¬„™â«‘∏’°“√∑“ß chromatography ∑’ˇ√’¬°«à“ herbal fin-  ‚¥¬«‘∏’°“√∑’Ëπ‘¬¡‰¥â·°à overlay and pattern matching ·≈–
                           5                                                                                6
             gerprint ‡ªìπÀ≈—° Õ¬à“߉√°Áμ“¡‡π◊ËÕß®“°§«“¡À≈“°À≈“¬  applied information content of fingerprint (√Ÿª∑’Ë 2)
             ¢Õßæ—π∏ÿåæ◊™ ·≈–∞“π¢âÕ¡Ÿ≈∑“ß«‘∑¬“»“ μ√å„π·μà≈–ª√–‡∑»    2. Quantitative method of chromatographic fin-

             ¡’§«“¡·μ°μà“ß°—π ∑”„Àâ¢âÕ°”Àπ¥·≈–«‘∏’°“√§«∫§ÿ¡     gerprint
             §ÿ≥¿“æ¢Õß«—μ∂ÿ¥‘∫·≈–¬“®“° ¡ÿπ‰æ√∑—Èß∑’ˇªì𬓇¥’ˬ«·≈–  ®–‡πâπ‰ª∑’Ë°“√·¬° (Separation) ·≈–°“√À“ª√‘¡“≥¢Õß
             ¬“μ”√—∫¡’§«“¡‡©æ“–‡®“–®ß·μ°μà“ß°—π‰ª„π·μà≈–ª√–‡∑»
                                                                     φ =-∫p /[sum( p )]log p /[sum( p )]dx
             „π∑’Ëπ’È®–¢Õ √ÿª«‘∏’°“√À≈—° Ê ∑’Ëπ‘¬¡„™â·≈–¢âÕ‡ πÕ∑’ˇÀ¡“–
              ¡ ”À√—∫ª√–‡∑»‰∑¬‡°’ˬ«°—∫°“√§«∫§ÿ¡§ÿ≥¿“æ«—μ∂ÿ¥‘∫  p = area under peak identified

             ·≈–¬“®“° ¡ÿπ‰æ√ ¥â«¬«‘∏’°“√ chromatographic finger-
                                                                √Ÿª∑’Ë 2 °“√§”π«π Applied information contents (φ)
             print ∑’Ëπ‘¬¡„™â ‰¥â·°à
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