Page 12 - journal-8-23-Full
P. 12
4 «“√ “√°“√·æ∑¬·º “√ ‰ ¬·≈– ° √·æ ∑“ß“ ∑¬ ªï∑’Ë 9©∫—∫∑’Ë 1 ¡°√“§¡- ‘ßÀ“§¡ 2554
“√°“√ ∑¬å·º ‰ ¬·≈–°“√ æ∑¬∑“å·ºπ‰∑¬·≈–°“√·æ∑¬å∑“߇≈◊Õ°
°“√ ∑¬åå·ºπ‰∑¬·≈–°“√·æ∑¬å∑“߇≈◊Õ°
«“√ “√°“√·æ∑¬
1. Thin Layer Chromatography: TLC
2. Gas Chromatography: GC
Safety §«“¡ª≈Õ¥¿—¬ ¬“®“° ¡ÿπ‰æ√ Efficacy ª√– ‘∑∏‘º≈ HPLC 3. High Performance Liquid Chromatography:
4. Electrophoretic Method
∑’Ë¥’
5. Spectrophotometry
Chromatographic fingerprint analysis
Quality §ÿ≥¿“æ
“¡“√∂·∫àßÕÕ°‰¥â‡ªìπ 2 ·∫∫§◊Õ °“√«‘‡§√“–À凙‘ß
√Ÿª∑’Ë 1 ≈—°…≥– concept ¢Õ߬“®“° ¡ÿπ‰æ√∑’Ë¥’ §ÿ≥¿“æ (qualitative method) ·≈–°“√«‘‡§√“–À凙‘ߪ√‘¡“≥
(quantitative method)
À≈—°‡°≥±å«‘∏’°“√∑’Ë¥’„π°“√º≈‘μ (Good Manufacturing 1. Qualitative method of chromatographic fin-
Practice À√◊Õ GMP) ¬“®“° ¡ÿπ‰æ√ ¡’°“√μ√«® Õ∫ ¡ÿπ gerprint
‰æ√«—μ∂ÿ¥‘∫∑’Ë„™â«à“‡ªìπ™π‘¥∑’Ë∂Ÿ°μâÕß ¡’ “√ ”§—≠„πª√‘¡“≥∑’Ë ¡’®ÿ¥ª√– ߧå„π°“√¥Ÿ√Ÿª·∫∫Õߧåª√–°Õ∫‚¥¬√«¡
μâÕß°“√ ‡¡◊ËÕºà“π°√–∫«π°“√º≈‘μ®π·≈⫇ √Á® °ÁμâÕß¡’°“√ (general characteristics) ‡æ◊ËÕ‡ª√’¬∫‡∑’¬∫§«“¡‡À¡◊Õπ
μ√«® Õ∫«à“¡’ “√ ”§—≠‡ªìπ‰ªμ“¡¡“μ√∞“π∑’Ë°”Àπ¥ (sameness) ·≈–§«“¡μà“ß (differences) ¢Õß«—μ∂ÿ¥‘∫·≈–
ªí®®ÿ∫—πÕÿμ “À°√√¡º≈‘μ¿—≥±å®“° ¡ÿπ‰æ√¡’°“√¢¬“¬ ¬“®“° ¡ÿπ‰æ√°—∫ “√¡“μ√∞“π‡æ◊ËÕ°“√æ‘ Ÿ®πå‡Õ°≈—°…≥å
μ—«Õ¬à“ß√«¥‡√Á« ‚¥¬¡’‚√ßß“πÕÿμ “À°√√¡¥—ß°≈à“«ª√–¡“≥ (identification) πÕ°®“°π’È “¡“√∂𔉪À“§«“¡ ¡Ë”‡ ¡Õ
2
1,117 ·Ààß (¢âÕ¡Ÿ≈ ≥ ¡’π“§¡ 2554) ‚¥¬¡’ —¥ à«π¢Õß‚√ßß“π ¢ÕßÕߧåª√–°Õ∫ (consistency) ¿“¬„π°“√º≈‘μ√ÿàπ‡¥’¬«°—π
3
„πμà“ß®—ßÀ«—¥¡“°°«à“„π°√ÿ߇∑未√–¡“≥ 2 ‡∑à“ ·μà¡’‡æ’¬ß ·≈–√–À«à“ß√ÿàπ∑’Ë°“√º≈‘μ ·≈–¬—ß𔉪„™â„π°“√μ√«® Õ∫
42 ·Àà߇∑à“π—Èπ∑’ˉ¥â√—∫°“√√—∫√Õß°“√§«∫§ÿ¡§ÿ≥¿“æ°“√º≈‘μ À“§«“¡§ßμ—« (stability) ‰¥â «‘∏’°“√π’È¡’¢âÕ¥’§◊Õ “¡“√∂
μ“¡¡“μ√∞“π “°≈ (Good Manufacturing Practice: GMP) §«∫§ÿ¡§ÿ≥¿“æ¢Õß«—μ∂ÿ¥‘∫·≈–¬“®“° ¡ÿπ‰æ√‰¥â·¡â«à“®–¬—ß
®“° ”π—°ß“π§≥–°√√¡°“√Õ“À“√·≈–¬“ °√–∑√«ß “∏“√≥ ÿ¢ ‰¡à¡’¢âÕ°”Àπ¥ “√¡“μ√∞“π∑’Ë®–π”¡“„™â„π°“√‡ª√’¬∫‡∑’¬∫
4
(¢âÕ¡Ÿ≈ ≥ ∏—𫓧¡ 2553) ¢≥–∑’Ë¢âÕ‡ ’¬¢Õß«‘∏’°“√π’ȧ◊Õ ‡°≥±å∑’Ë„™â„π°“√ª√–‡¡‘πº≈
πÕ°®“°π’ÈÕߧ尓√Õπ“¡—¬‚≈° (World Health Organi- °“√«‘‡§√“–Àå·≈–°“√°”Àπ¥§à“¡“μ√∞“π¬—߉¡à¡’§«“¡™—¥‡®π
zation: WHO) ‰¥â°”À𥫑∏’°“√§«∫§ÿ¡§ÿ≥¿“æ¢Õß«—μ∂ÿ¥‘∫ ¡’§«“¡¬ÿà߬“° ´—∫´âÕπ ·≈–·μ°μà“ß°—π‰ª„π·μà≈–ª√–‡∑»
∑’ˇªìπæ◊™ ¡ÿπ‰æ√∑’Ëπ”¡“∑”‡ªì𬓷≈–º≈‘μ¿—≥±å®“° ¡ÿπ à«π„À≠à®–¢÷Èπ°—∫™π‘¥¢Õ߇§√◊ËÕß¡◊Õ·≈– software ∑’Ë„™â
‰æ√‚¥¬„™â«‘∏’°“√∑“ß chromatography ∑’ˇ√’¬°«à“ herbal fin- ‚¥¬«‘∏’°“√∑’Ëπ‘¬¡‰¥â·°à overlay and pattern matching ·≈–
5 6
gerprint ‡ªìπÀ≈—° Õ¬à“߉√°Áμ“¡‡π◊ËÕß®“°§«“¡À≈“°À≈“¬ applied information content of fingerprint (√Ÿª∑’Ë 2)
¢Õßæ—π∏ÿåæ◊™ ·≈–∞“π¢âÕ¡Ÿ≈∑“ß«‘∑¬“»“ μ√å„π·μà≈–ª√–‡∑» 2. Quantitative method of chromatographic fin-
¡’§«“¡·μ°μà“ß°—π ∑”„Àâ¢âÕ°”Àπ¥·≈–«‘∏’°“√§«∫§ÿ¡ gerprint
§ÿ≥¿“æ¢Õß«—μ∂ÿ¥‘∫·≈–¬“®“° ¡ÿπ‰æ√∑—Èß∑’ˇªì𬓇¥’ˬ«·≈– ®–‡πâπ‰ª∑’Ë°“√·¬° (Separation) ·≈–°“√À“ª√‘¡“≥¢Õß
¬“μ”√—∫¡’§«“¡‡©æ“–‡®“–®ß·μ°μà“ß°—π‰ª„π·μà≈–ª√–‡∑»
φ =-∫p /[sum( p )]log p /[sum( p )]dx
„π∑’Ëπ’È®–¢Õ √ÿª«‘∏’°“√À≈—° Ê ∑’Ëπ‘¬¡„™â·≈–¢âÕ‡ πÕ∑’ˇÀ¡“–
¡ ”À√—∫ª√–‡∑»‰∑¬‡°’ˬ«°—∫°“√§«∫§ÿ¡§ÿ≥¿“æ«—μ∂ÿ¥‘∫ p = area under peak identified
·≈–¬“®“° ¡ÿπ‰æ√ ¥â«¬«‘∏’°“√ chromatographic finger-
√Ÿª∑’Ë 2 °“√§”π«π Applied information contents (φ)
print ∑’Ëπ‘¬¡„™â ‰¥â·°à