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weight, food consumption, relative organ weight and blood chemistry values. The increases

of neutrophil in male rats receiving the extract at the dose 12.0 and 60.0 mg/kg/day
were minor change and still within normal range. Necropsy results showed no gross
pathological changes of visceral organs. Histopathology revealed no abnormalities in any
organs except male rats receiving the highest dose of extract had higher incidence of mild

fatty change. Therefore, application of the extract for coloring agent at high concentration
or overdose should be avoided and the amount of active ingredients (bixin and norbixin) in

the extract should be taken into account for appropriate concentration in each food stuff as
reported in other countries.

Key words : Bixa orellina, Toxicity

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