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º≈¢Õß “√ °—¥®“°„∫¡–√ÿ¡ (Moringa oleifera) μàÕ°“√· ¥ßÕÕ°¢Õ߬’π
IDH1/IDH2 „π‡´≈≈å “¬æ—π∏ÿå¡–‡√Á߇¡Á¥‡≈◊Õ¥¢“«™π‘¥ U937

‰™¬«—≤πå ‰™¬ ¡∫Ÿ√≥å, π∑’≈—°…≥å °ÿ≈‡∑», °“≠®π“ ÕŸà ÿ«√√≥∑‘¡

Àπ૬«‘®—¬¿Ÿ¡‘§ÿâ¡°—π√–¥—∫‡´≈≈å·≈–‚¡‡≈°ÿ≈ §≥– À‡«™»“ μ√å ¡À“«‘∑¬“≈—¬π‡√»«√ ®—ßÀ«—¥æ‘…≥ÿ‚≈°

À≈—°°“√·≈–‡Àμÿº≈ ¡–‡√Á߇¡Á¥‡≈◊Õ¥¢“«™π‘¥¡—¬Õ’≈Õ¬¥å·∫∫‡©’¬∫æ≈—π ¡’Õÿ∫—μ‘°“√≥å Ÿß¢÷Èπ„πºŸâ ŸßÕ“¬ÿ
·≈–ºŸâªÉ«¬¡—°¡’Õ“°“√∑√ÿ¥Àπ—°„π√–¬–‡«≈“Õ—π√«¥‡√Á« ®“°°“√»÷°…“∑’˺à“π¡“æ∫«à“ “√ °—¥®“°„∫¡–√ÿ¡¡’
“√°≈ÿà¡øï‚π≈‘°´÷Ëß¡’§ÿ≥ ¡∫—μ‘„π°“√μâ“π¡–‡√Áß “¡“√∂°√–μÿâπ„À⇰‘¥°“√쓬¢Õ߇´≈≈å¡–‡√Áß ·μà√“¬ß“π
§«“¡‡ªìπæ‘…¢Õß “√ °—¥¡–√ÿ¡°—∫‡´≈≈å¡–‡√Á߇¡Á¥‡≈◊Õ¥¢“«¬—ß¡’‰¡à¡“° Õ’°∑—È߬—߉¡à¡’√“¬ß“π∂÷ߺ≈¢Õß “√

°—¥„∫¡–√ÿ¡μàÕ°“√· ¥ßÕÕ°¢Õ߬’π∑’ˇ°’ˬ«¢âÕß°—∫°“√§«∫§ÿ¡°“√‡°‘¥¡–‡√Á߇¡Á¥‡≈◊Õ¥¢“«„π√–¥—∫æ—π∏ÿ°√√¡
«—μ∂ÿª√– ß§å ‡æ◊ËÕ»÷°…“ƒ∑∏‘Ï¢Õß “√ °—¥„∫¡–√ÿ¡μàÕ‡´≈≈å “¬æ—π∏ÿå¡–‡√Á߇¡Á¥‡≈◊Õ¥¢“«‡æ“–‡≈’Ȭߙπ‘¥
U-937 μàÕ°“√· ¥ßÕÕ°¢Õ߬’π IDH1 ·≈– IDH2

«‘∏’¥”‡π‘π°“√ π” “√ °—¥„∫¡–√ÿ¡„π™—Èπ‡Õ∑‘≈Õ–´‘‡μμ¡“»÷°…“§«“¡‡ªìπæ‘…¥â«¬«‘∏’ Neutral red as-
say ·≈–μ√«®√–¥—∫°“√· ¥ßÕÕ°¢Õ߬’π IDH1 ·≈– IDH2 ‚¥¬«‘∏’ quantitative real-time polymerase

chain reaction (qRT-PCR) ‚¥¬„™â‡´≈≈å‚¡‚π‰´μå∑’Ë·¬°®“°¡πÿ…¬å‡ªìπ‡´≈≈姫∫§ÿ¡·≈–‡´≈≈å¡–‡√Á߇¡Á¥
‡≈◊Õ¥¢“«™π‘¥ U-937 ·≈–„™â¬“ azacitidine ‡ªìπ “√§«∫§ÿ¡∫«°

º≈°“√»÷°…“ “√ °—¥„∫¡–√ÿ¡„π™—Èπ‡Õ∑‘≈Õ–´‘‡μμ∑’˧«“¡‡¢â¡¢âπ´÷Ë߇ªìπæ‘…μàÕ‡´≈≈å°—∫°“√· ¥ßÕÕ°¢Õß
¬’π IDH1 ·≈– IDH2 æ∫«à“ “√ °—¥®“°„∫¡–√ÿ¡„𧫓¡‡¢â¡¢âπ∑’Ë∑”„À⇴≈≈嬗ߡ’™’«‘μ√âÕ¬≈– 95 (lethal con-
centration 5, LC ) ¡’ƒ∑∏‘Ï„π°“√°√–μÿâπ°“√· ¥ßÕÕ°¢Õ߬’π‰¥â¡“°°«à“ “√ °—¥„∫¡–√ÿ¡„𧫓¡‡¢â¡¢âπ∑’Ë∑”„Àâ
5
‡´≈≈嬗ߡ’™’«‘μ√âÕ¬≈– 90 (LC ) ‚¥¬ “√ °—¥„∫¡–√ÿ¡ “¡“√∂‡æ‘Ë¡°“√· ¥ßÕÕ°¢Õ߬’π IDH1 ·≈– IDH2 „π
10
‡´≈≈å U937

¢âÕ √ÿª “√ °—¥„∫¡–√ÿ¡„π™—Èπ‡Õ∑‘≈Õ–´‘‡μμ “¡“√∂‡æ‘Ë¡°“√· ¥ßÕÕ°¢Õ߬’π IDH1 ·≈– IDH2 ‰¥â
‡™àπ‡¥’¬«°—∫¬“ azacitidine ™’È„Àâ‡ÀÁπ«à“ “√ °—¥π’È¡’·π«‚πâ¡∑’Ë®–𔉪æ—≤π“‡æ◊ËÕπ”¡“„™â√à«¡°—∫°“√√—°…“‚√§

¡–‡√Á߇¡Á¥‡≈◊Õ¥¢“«‰¥â ·μଗßμâÕß¡’°“√»÷°…“‡æ‘Ë¡‡μ‘¡μàÕ‰ª