Page 24 - วารสารกรมการแพทย์แผนไทยฯ ปีที่ 10 ฉบับที่ 2

100 «“√ “√°“√·æ∑¬å·ºπ‰∑¬·≈–°“√·æ∑¬å∑“߇≈◊Õ° ªï∑’Ë 10 ©∫—∫∑’Ë 2 情¿“§¡- ‘ßÀ“§¡ 2555

Discussion and Conclusion phase may help to separate acidic compounds

In this study, the samples were prepared in the samples.15 The chromatogram of HNK
based on the traditional usage of Thai tradi- revealed that the complexity of recipes could
tional herbal remedies in form of decoction lead to an unclear separation. This undesir-
or herbal tea. However, due to difficulty in able result might be attributed to the mul-
dissolving AA-I in water, ammonia was used tiple components of many herb species
in the sample preparation. Specimens of KK blended together in the recipe. Therefore, to
and a Thai herbal recipe (HNK) were, there- improve the AA-I detection in multiple herbal
fore, extracted with tow kinds of solvents, recipe, the sample should be cleaned before
ammonia and water. The results showed that the analysis to remove the interfering com-

the Rf value and absorption spectrum of AA- pounds.
I were similar for the samples extracted with The method developed in this study is
either ammonia or water. The intensity of AA- a primary tool for rapid identification of AA-
I peak of ammonia extract was higher than I in herbal materials to ensure their safety on
that of water extract. the basis of the absence of AA. However, to
HPTLC and RP-TLC analyses could de- complete the qualitative and quantitative
tect AA-I in a single herb but not in a mul- analyses of AA in the recipe containing KK,

tiple herbal recipe. This may be due to the further development should be performed in
complexity of a lot of ingredients in the recipe. the step of sample purification to obtain a
Although several mobile phase systems were better resolution of AA chromatogram.
tried in this study to obtain the best separa-
Acknowledgements
tion for the identification of AA-I in KK speci-
men and one Thai herbal recipe containing We would like to thank Assoc. Prof.
KK, the peak of AA-I was isolated and de- Rungravi Temsiririrkkul, Department of Phar-
tected only in the KK specimen but not in maceutical Botany, Faculty of Pharmacy,
the recipe. The best solvent system for HPTLC Mahidol University, Thailand for her sugges-

analysis was chloroform/methanol/acetic acid tions.
(65:20:1, v/v) while the best solvent system
for RP-TLC analysis was acetonitrile/metha-
References
nol/water (3:0.5:1, v/v). The lower limits of
1. Tian-Shung W, Li-Fei O, Che-Ming T. Aristolochic acids,
detection values of AA-I were found in nano-
aristolactam alkaloids and amides from Aristolochia
gram, which were 8 ng and 15 ng for HPTLC kankauensis. Phytochemistry 1994;36:1063-8.
and RP-TLC, respectively. The HPTLC analy- 2. Yuan J, Nie L, Zeng D, Luo X, Tang F, Ding L, et al.
Simultaneous determination of nine aristolochic acid
sis revealed better sensitivity than that us-
analogues in medicinal plants and preparations by high-
ing the RP-TLC method. The addition of a
performance liquid chromatography. Talanta 2007;73:644-
small amount of acetic acid in the mobile 50.