Page 23 - วารสารกรมการแพทย์แผนไทยฯ ปีที่ 10 ฉบับที่ 2

Journal of Thai Traditional & Alternative Medicine Vol. 10 No. 2 May-August 2012 99

Fig. 5 Chromatogram of KK and HNK in RP-TLC analysis detected under UV 323 nm. Absorption spectrum
scanned from wavelength 200-450 nm was demonstrated in the right corner of each chromatogram.

sharp peak (Figure 3). tion than that of HNK. Although the peak
with the Rf value similar to that of the mark-
Identification of aristolochic acids in HNK
ers was exhibited in both KK and HNK, the
using HPTLC analysis
absorption spectrum of the peak of the
Although the chromatogram of HNK in recipe was not similar to those of the mark-
all mobile phase systems showed a suspected ers (Figure 5).
peak that had the Rf value near to the AA-I
Lower limit of detection (LOD)
marker; however, their absorption spectra
were not similar to those of the markers By diluting the concentration of AA-I
(Figure 4). and AA-I salt until the lowest concentration
yielded the average response approximately
Identification of aristolochic acid compound
3 times as high as the baseline noise level,
in KK and HNK by RP-TLC analysis
HPTLC analysis had the lower limit of detec-
Using RP-TLC with solvent system of tion at 8 ng for AA-I and 5 ng for AA-I salt.

acetonitrile/methanol/water (3:0.5:1) give the For RP-TLC analysis, the lower limits detected
results corresponding to HPTLC analysis. for both AA-I and AA-I salt were 15 ng.
Chromatogram of KK showed better separa-