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Journal of Thai Traditional & Alternative Medicine                Vol. 6 No. 2 May-August (Supplement) 2008 Ù˜



                OR-7



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             Õ‘π∑—™ »—°¥‘Ï¿—°¥’‡®√‘≠ , Õ√ÿ≥æ√ Õ‘∞√—µπå
             Ò
             §≥–·æ∑¬»“ µ√å ¡À“«‘∑¬“≈—¬∏√√¡»“ µ√å
             Ú
              »Ÿπ¬å°“√·æ∑¬å·ºπ‰∑¬ª√–¬ÿ°µå §≥–·æ∑¬»“ µ√å ¡À“«‘∑¬“≈—¬∏√√¡»“ µ√å


             À≈—°°“√·≈–‡Àµÿº≈ „πªí®®ÿ∫—π‚√§¡–‡√Áßπ—∫«à“‡ªìπªí≠À“∑“ß “∏“√≥ ÿ¢∑’Ë ”§—≠¡“° ∑—Èß„π√–¥—∫‚≈°·≈–„π√–¥—∫
             ª√–‡∑» ®“°°“√»÷°…“¿Ÿ¡‘ªí≠≠“°“√„™â ¡ÿπ‰æ√„π°“√√—°…“‚√§¡–‡√ÁߢÕßÀ¡Õæ◊Èπ∫â“π¿“§„µâ æ∫«à“¡’°“√„™â
              ¡ÿπ‰æ√À≈“¬™π‘¥„π°“√√—°…“‚√§¡–‡√Áß‚¥¬‡©æ“–æ‘°—¥‡∫≠®°Ÿ≈ ´÷Ëߪ√–°Õ∫¥â«¬ ¡ÿπ‰æ√ ı ™π‘¥ §◊Õ º≈¥’ª≈’
             (Piper longum «ß»å Piperaceae), √“°™â“æ≈Ÿ (Piper sarmentosum «ß»å Piperaceae), ‡∂“ –§â“π (Piper rostratum «ß»å
             Piperaceae), ‡Àßâ“¢‘ß·Àâß (Zingiber officinale «ß»å Zingiberaceae) ·≈–√“°‡®µ¡Ÿ≈‡æ≈‘ß·¥ß (Plumbago indica «ß»å

             Plumbaginaceae) ‚¥¬®–„™â‡ªì𬓪√—∫∏“µÿ„πºŸâªÉ«¬¡–‡√Áß°àÕπ°“√√—°…“¥â«¬¬“™π‘¥Õ◊Ëπ

             «—µ∂ÿª√– ß§å ‡æ◊ËÕ»÷°…“ƒ∑∏‘ϧ«“¡‡ªìπæ‘…µàÕ‡´≈≈å¡–‡√ÁߢÕß “√ °—¥‡Õ∑“πÕ≈¢Õß ¡ÿπ‰æ√„πæ‘°—¥‡∫≠®°Ÿ≈

             «‘∏’¥”‡π‘π°“√ π” “√ °—¥∑—ÈßÀ¡¥¡“∑¥ Õ∫ƒ∑∏‘ϵâ“π‡´≈≈å¡–‡√Á߇µâ“π¡ (MCF-7) ªÕ¥ (COR-L23) µ—∫ (HepG2) ·≈–
             ª“°¡¥≈Ÿ° (Hela) ‚¥¬‡´≈≈å‡À≈à“π’È®–π”¡“‡≈’Ȭ߄πÕ“À“√‡≈’Ȭ߇´≈≈å∑’ˇÀ¡“– ¡ ∑”°“√∂à“¬‡´≈≈å≈ß„π 96-well plate
             𔉪‡≈’Ȭ߄π CO  incubator ‡ªìπ‡«≈“ ÚÙ ™—Ë«‚¡ß ‡µ‘¡ “√≈–≈“¬µ—«Õ¬à“ߧ«“¡‡¢â¡¢âπµà“ß Ê ·≈â«π”‰ª‡≈’Ȭ߄π CO
                           2                                                                                   2
             incubator µàÕ‡ªìπ‡«≈“ ˜Ú ™—Ë«‚¡ß ®“°π—Èπ∑”°“√≈â“߇´≈≈å·≈â«π”‰ª‡≈’Ȭ߄π CO  incubator µàÕÕ’°‡ªìπ‡«≈“ ˜Ú ™—Ë«‚¡ß
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             ‡æ◊ËÕ¥ŸÕ—µ√“°“√√Õ¥¢Õ߇´≈≈å¡–‡√Áß‚¥¬«‘∏’ SRB assay (sulphorhodamine B assay) ‚¥¬°“√ fix ‡´≈≈å∑’Ë¡’™’«‘µ¥â«¬ Ù
             ‡ªÕ√凴Áπµå trichloroacetic acid ·≈–¬âÕ¡‡´≈≈å¥â«¬ ’ SRB ®“°π—Èπ®÷ß≈–≈“¬ ’¥â«¬ trisma base ·≈â««—¥§à“°“√¥Ÿ¥°≈◊π
             · ß UV ¥â«¬ microplate reader ∑’˧«“¡¬“«§≈◊Ëπ Ù˘Ú π“‚π‡¡µ√ π”§à“∑’ˉ¥â¡“§”π«≥§à“∑“ß ∂‘µ‘·≈–À“§à“ IC
                                                                                                              50
             ¥â«¬ prism program

             º≈°“√»÷°…“ æ∫«à“ æ‘°—¥‡∫≠®°Ÿ≈∑’Ë °—¥¥â«¬‡Õ∑“πÕ≈ÕÕ°ƒ∑∏‘ϵâ“π¡–‡√Áߪե¥’∑’Ë ÿ¥ (IC  = Ò˘.¯ ‰¡‚§√°√—¡/¡‘≈≈‘≈‘µ√)
                                                                                     50
             √Õß≈ß¡“§◊Õ  “¡“√∂ÕÕ°ƒ∑∏‘ϵâ“π¡–‡√Á߇µâ“π¡ (IC  = ÛÛ.Ú ‰¡‚§√°√—¡/¡‘≈≈‘≈‘µ√) ¡’ƒ∑∏‘Ϫ“π°≈“ß°—∫¡–‡√Áߪ“°
                                                        50
             ¡¥≈Ÿ°·≈–¡–‡√Áßµ—∫  à«πæ◊™∑’ˇªìπ à«πª√–°Õ∫¢Õßæ‘°—¥‡∫≠®°Ÿ≈·≈–¡’º≈µàÕ¡–‡√Áߪե¥’∑’Ë ÿ¥§◊Õ ‡®µ¡Ÿ≈‡æ≈‘ß·¥ß (IC 50
             = Û.Ù ‰¡‚§√°√—¡/¡‘≈≈‘≈‘µ√) √Õß≈ß¡“§◊Õ ¢‘ß (IC = ˜.˘ ‰¡‚§√°√—¡/¡‘≈≈‘≈‘µ√) ·≈–‡¡◊ËÕ∑¥ Õ∫°—∫‡´≈≈åªÕ¥ª°µ‘
                                                       50
             æ∫«à“  “√ °—¥‡∫≠®°Ÿ≈¥â«¬‡Õ∑“πÕ≈ÕÕ°ƒ∑∏‘Ï¶à“‡´≈≈åªÕ¥ª°µ‘‰¥âπâÕ¬

             ¢âÕ √ÿª  “√ °—¥¥â«¬‡Õ∑“πÕ≈¢Õßæ‘°—¥‡∫≠®°Ÿ≈¡’§«“¡‡ªìπæ‘…µàÕ‡´≈≈å¡–‡√Áß ‚¥¬¡’§«“¡®”‡æ“–‡®“–®ßµàÕ‡´≈≈å¡–‡√Áß

             ªÕ¥¡“°∑’Ë ÿ¥ πÕ°®“°π’Ȭ—ß¡’§«“¡‡ªìπæ‘…µàÕ‡´≈≈åªÕ¥ª°µ‘πâÕ¬Õ’°¥â«¬
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