Page 46 - journal_13-3_Full

230

2. Nualkaew S, Gritsanapan W, Petereit F, Nahrstedt A. New cassumunar and Nigellar sativa. Chinese Medicine.
fatty acid esters originate during storage by the interaction 2009;4:19.
of components in Prasaplai, a Thai traditional medicine. 6. Neises B, Steglich W. Simple method for the esterification
Planta Medica. 2004;70:1243-6. of carboxylic acids. Angewandte Chemie International
3. Tangyuenyongwatana P, Gritsanapan. Mechanism Edition. 1978;17:522-4.
evaluation of artifacts formation in Prasaplai preparation, a 7. Abramovic H, Jamnik M, Burkan L, Kac M. Water activity
Thai traditional medicine. Planta Medica. 2008;74:1403-5. and water content in Slovenian honeys. Food Control.
4. Tangyuenyongwatana P, Gritsanapan W. Quantitative 2008;19:1086-90.
analysis and toxicities determination of artifacts originated 8. Flamini C, Morlot M, Lheretier J, Borneck R, Marioleas P,
in a Thai traditional medicine Prasaplai. Pharmaceutical Tsigouri A, et al. Honey quality, method of analysis and
Biolology. 2010;48(5):584-8. international regulatory standards: Review of the work of
5. Tangyuenyongwatana P, Gritsanapan W, Kowapradit J, the international honey commission. Swiss Bee Research
Opanasopit P. Cellular transport of anti-inflammatory pro- Centre. 2000;5:1-15.
drugs originated from a herbal formulation of Zingiber

Abstract

A Quantitative Study on Artifacts Occurring in Prasa-Plai Preparation in the Pill and
Capsule Forms
,‡
Somnuek Suchaitanawanich* , Prasan Tangyuenyongwatana †
*Herb and Thai Traditional Medicine Division, Department of Thai Traditional and Complementary
Medicine, Ministry of Public Health, Nonthaburi 11000
†
College of Oriental Medicine, Rangsit University, Pathumthani 12000
‡
Corresponding author: somnuekster@gmail.com
This study aimed to compare the amounts of artifacts that occurred in two Prasa-Plai (Thai
traditional medicine for dysmenorrhea) preparations: pill form and capsule form. Four re-
ference standard compounds (Compound D, Artifact 1, Artifact 2, and Artifact 3) were synthe-
sized using a chemical method. And, based on the four compounds, a quantitative analysis method
with a high level of accuracy and precision was developed using high performance liquid chro-
matography (HPLC). Three dosage forms of Prasa-Plai were prepared: capsule form, pill with
honey form and pill with simmered honey form. In 49 days, the drugs in all three forms were
analyzed. It was found that the amounts of artifacts of Prasa-Plai in pill with honey and pill with
simmered honey forms were 1.57-2.07 times and 1.86-2.60 times, respectively, less than that in the
capsule form, because the humidity in the pill forms could slow down the esterification reaction.
There was no significant difference in the amounts of artifacts in the pill with honey and the pill
with simmered honey forms (p<0.01). Thus, to avoid a higher amount of artifacts in Prasa-Plai
with unknown activities, one should use the pill form either with honey or simmered honey.
Key words: Prasa-Plai, artifacts, pill, capsule