Page 41 - journal_13-3_Full
P. 41

225




              ‰¥âº≈º≈‘μ√âÕ¬≈– 62.38, 71.40 ·≈– 65.65 μ“¡≈”¥—∫  „μâ°√“ø¢Õß “√·μà≈–™π‘¥ „À⇪ìπ§à“§«“¡‡¢â¡¢âπ

              ‚¥¬√“¬≈–‡Õ’¬¥¢Õß°“√∑¥≈Õß·≈–¢âÕ¡Ÿ≈∑“ß ‡ª°-  ¢Õß “√π—Èπ Ê ®“° ¡°“√‡ âπμ√ߢÕß “√¡“μ√∞“π
                                                     3                   2
              ‚∑√ ‚°ªï Õ¥§≈âÕß°—∫º≈°“√∑¥≈Õß∑’ˇ§¬√“¬ß“π  ∑—Èß ’Ë™π‘¥æ∫«à“ §à“ r  ¢Õß “√¡“μ√∞“π  “√ D °—∫
                  2. °“√À“ª√‘¡“≥πÈ”„ππÈ”º÷Èß∑’Ëπ”¡“‡μ√’¬¡¬“   “√ 1, 2 ·≈– 3 ¡’§à“‡∑à“°—∫ 0.9999 ¥—ßμ“√“ß∑’Ë 2

              ≈Ÿ°°≈Õπ                                    ´÷Ëß∂◊Õ«à“‡ªìπ§à“∑’Ë¥’ ¡’§«“¡·¡à𬔠Ÿß∑—Èß∑’˧«“¡‡¢â¡
                     ‚¥¬°“√«—¥¥—™π’À—°‡À¢ÕßπÈ”º÷Èß ·≈â«  ¢âπμË”·≈–§«“¡‡¢â¡¢âπ Ÿß
              §”π«≥‡ªìπª√‘¡“≥¢ÕßπÈ”∑’Ë¡’Õ¬Ÿà„ππÈ”º÷Èß æ∫«à“πÈ”  3.2 Accuracy §à“§«“¡∂Ÿ°μâÕߢÕß°“√∑”°“√

              º÷Èß∏√√¡¥“ ·≈–πÈ”º÷Èß∑’ˇ§’ˬ«¡’ª√‘¡“≥πÈ”‡∑à“°—∫  «‘‡§√“–Àå¢Õ߬“μ”√—∫ª√– –‰æ≈ ‚¥¬„™â°“√‡μ‘¡ “√
              √âÕ¬≈– 20.67 ± 0.06 ·≈– 19.33 ± 0.06 μ“¡≈”¥—∫  ¡“μ√∞“π∑’Ë∑√“∫ª√‘¡“≥∑’Ë·πàπÕπ≈߉ª„πμ—«Õ¬à“ß

                  3. °“√æ—≤π“«‘∏’«‘‡§√“–Àåª√‘¡“≥  “√ D °—∫   “√ °—¥ ·≈â«∑”°“√À“ % recovery ¢Õß “√
               “√ 1, 2 ·≈– 3 ‰¥â HPLC ‚§√¡“‚∑·°√¡¥—ß√Ÿª∑’Ë 4  ¡“μ√∞“π∑’Ë‡μ‘¡π—Èπ §à“§«“¡‡¢â¡¢âπ¢Õß “√∑’ˉ¥â®“°
              ·≈–‰¥âº≈°“√∑¥≈Õߥ—ßμàÕ‰ªπ’È                ‡§√◊ËÕß π”¡“§”π«≥§à“ % recovery ‚¥¬„™â Ÿμ√

                     3.1 Linearity ·≈– Range: ®—¥∑”°√“ø       % recovery = (C  - C )/ C * × 100
                                                                            C    A   A
              ¡“μ√∞“π·≈–°”Àπ¥™à«ß∑’Ë„™â„π°“√«‘‡§√“–Àå         ‚¥¬∑’Ë C  = §«“¡‡¢â¡¢âπ∑’Ë«—¥‰¥â¢Õßμ—«Õ¬à“ß
                                                                     C
                   ¡°“√‡ âπμ√ߢÕß°√“ø¡“μ√∞“π·≈–§à“ cor-  ∑’˺à“π°“√ spike ·≈â«
                               2
              relation coefficient (r ) ¡’ §«“¡ ”§—≠μàÕ°“√∑”°“√     C  = §«“¡‡¢â¡¢âπ∑’Ë«—¥‰¥â¢Õßμ—«Õ¬à“ß
                                                                     A
              «‘‡§√“–Àåª√‘¡“≥ ‡π◊ËÕß®“°‡ªìπ ¡°“√§≥‘μ»“ μ√å∑’Ë  ∑’ˉ¡à‰¥â∑”°“√ spike
              ®–„™â„π°“√·ª≈º≈°“√«‘‡§√“–À宓°§à“¢âÕ¡Ÿ≈æ◊Èπ∑’Ë        C * = §«“¡‡¢â¡¢âπ¢Õß “√∑’ˇªìπ spike
                                                                     A
              μ“√“ß∑’Ë 2  √ÿª§à“ linearity ¢Õß “√ D °—∫ “√ 1, 2 ·≈– 3

               “√                  ¡°“√‡ âπμ√ß           —¡ª√– ‘∑∏‘Ï À —¡æ—π∏å (r )   Range (ppm.)
                                                                           2
               “√ D            y = 3E-05x - 0.2481             0.9999                  4 - 470
               “√ 1            y = 9E-05x + 1.2786             0.9999                  10 - 500
               “√ 2            y = 7E-05x - 0.0258             0.9999                   4 - 450
               “√ 3            y = 9E-05x + 0.1825             0.9999                   4 - 500


              μ“√“ß∑’Ë 3 §à“ %recovery ∑’Ë√–¥—∫§«“¡‡¢â¡¢âπμË” (low)

                                 Control sample    Add       Found (§«“¡‡¢â¡¢âπ∑’Ë«—¥‰¥â)
               “√¡“μ√∞“π                                                               (%recovery)
                                    (ppm)         (ppm)              (ppm)
               “√ D                130.12         35.00             165.55               101.23
               “√ 1                142.57         37.50             180.82               102.00
               “√ 2                 32.64         33.50               66.09               99.85
               “√ 3                 60.39         36.75               96.62               98.58
   36   37   38   39   40   41   42   43   44   45   46