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             ‡¡∑“πÕ≈ 0.2 ¡‘≈≈‘≈‘μ√ ‡¢¬à“„À⇢⓰—π      ‡ªìπ§à“ relative standard deviation (RSD) ¢Õß

                    3.5 ¢«¥∑’Ë 5- Concentration 3: ªî‡ªμ  °“√∑”´È”∑—ÈßÀ¡¥
              “√≈–≈“¬¡“μ√∞“π¢Õß  “√ D  “√ 1, 2 ·≈– 3            2.3 Limit of Detection (LOD) ‡ªìπ°“√
             ª√‘¡“μ√ 0.4 ¡‘≈≈‘≈‘μ√ æ√âÕ¡∑—Èߪμ stock solu-  À“ª√‘¡“≥∑’ËμË”∑’Ë ÿ¥∑’Ë “¡“√∂μ√«®«—¥‰¥â®“°‡§√◊ËÕß

             tion ¢Õ߬“ª√– –‰æ≈ª√‘¡“μ√ 1 ¡‘≈≈‘≈‘μ√ ≈ß„π  ¡◊Õ∑’Ë„™â ∑”‰¥â‚¥¬‡®◊Õ®“ß “√¡“μ√∞“π ®π‰¥â§à“ sig-
             eppendrof ¢π“¥ 1.5 ¡‘≈≈‘≈‘μ√ ‡¢¬à“„À⇢⓰—π  nal/noise ratio ‡∑à“°—∫ 3: 1 Limit of Quantitation
                 °√Õßμ—«Õ¬à“ߢÕß “√≈–≈“¬∑’Ë®–∑”°“√      (LOQ) §◊Õ§«“¡‡¢â¡¢âπ∑’ËμË”∑’Ë ÿ¥∑’Ë«‘∏’«‘‡§√“–Àå

             «‘‡§√“–Àå¥â«¬ nylon filter membrane ¢π“¥ 0.45   “¡“√∂μ√«®«—¥‰¥âÕ¬à“ß∂Ÿ°μâÕß ∑”‰¥â‚¥¬‡®◊Õ®“ß
             ‰¡§√Õπ °àÕπ∑”°“√«‘‡§√“–Àå¥â«¬‡§√◊ËÕß HPLC §à“   “√¡“μ√∞“π ®π‰¥â§à“ signal/noise ratio ‡∑à“°—∫

             §«“¡‡¢â¡¢âπ¢Õß “√∑’ˉ¥â®“°‡§√◊ËÕß π”¡“§”π«≥§à“  10 : 1
             % recovery
                                                                      º≈°“√»÷°…“
                    2.2 Precision ‡ªìπ°“√μ√«® Õ∫§«“¡

             ‡∑’ˬßμ√ߢÕß«‘∏’«‘‡§√“–Àå ‡æ◊ËÕ¥Ÿ«à“º≈°“√«‘‡§√“–Àå∑’Ë  1. °“√‡μ√’¬¡ “√¡“μ√∞“π  “√ D °—∫ “√ 1,
             ∑”´È”„π·μà≈–§√—Èß ‚¥¬°“√©’¥ “√¡“μ√∞“π·μà≈–  2 ·≈– 3

             ™π‘¥∑’˧«“¡‡¢â¡¢âπ√–¥—∫°≈“ߢÕß linearity ®”π«π   “√¡“μ√∞“π∑—Èß 4 ™π‘¥ ‰¥â·°à  “√ D °—∫ “√
             6 §√—ÈßμàÕ‡π◊ËÕß°—π ‡√’¬°«à“ intra-day precision  1, 2 ·≈– 3 ‰¥â®“°«‘∏’‡§¡’ —߇§√“–Àå ‚¥¬‰¥âº≈º≈‘μ
             ·≈–π” “√¡“μ√∞“π‡¥‘¡©’¥´È”Õ’° 6 §√—Èß„π«—πμàÕ¡“  ¢Õß “√·μà≈–™π‘¥¥—ßπ’È  “√ D ‰¥âº≈º≈‘μ‡∑à“°—∫

             π”§à“∑—Èß Õß«—π¡“§”π«≥ ‡√’¬°«à“ inter-day pre-  √âÕ¬≈– 28.27 ‚¥¬ºà“π°“√ —߇§√“–Àå 4 ¢—ÈπμÕπ  “√
             cision §à“ precision ¢Õß«‘∏’«‘‡§√“–Àå¡—°§”π«≥  1, 2 ·≈– 3 ‡°‘¥®“°ªØ‘°‘√‘¬“ Steglich reaction





























                     √Ÿª∑’Ë 4 HPLC ‚§√¡“‚μ·°√¡¢Õß “√¡“μ√∞“π  “√ D °—∫ “√ 1(A1), 2(A2) ·≈– 3(A3)
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