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‡¡∑“πÕ≈ 0.2 ¡‘≈≈‘≈‘μ√ ‡¢¬à“„À⇢⓰—𠇪ìπ§à“ relative standard deviation (RSD) ¢Õß

3.5 ¢«¥∑’Ë 5- Concentration 3: ªî‡ªμ °“√∑”´È”∑—ÈßÀ¡¥
“√≈–≈“¬¡“μ√∞“π¢Õß “√ D “√ 1, 2 ·≈– 3 2.3 Limit of Detection (LOD) ‡ªìπ°“√
ª√‘¡“μ√ 0.4 ¡‘≈≈‘≈‘μ√ æ√âÕ¡∑—Èߪμ stock solu- À“ª√‘¡“≥∑’ËμË”∑’Ë ÿ¥∑’Ë “¡“√∂μ√«®«—¥‰¥â®“°‡§√◊ËÕß

tion ¢Õ߬“ª√– –‰æ≈ª√‘¡“μ√ 1 ¡‘≈≈‘≈‘μ√ ≈ß„π ¡◊Õ∑’Ë„™â ∑”‰¥â‚¥¬‡®◊Õ®“ß “√¡“μ√∞“π ®π‰¥â§à“ sig-
eppendrof ¢π“¥ 1.5 ¡‘≈≈‘≈‘μ√ ‡¢¬à“„À⇢⓰—π nal/noise ratio ‡∑à“°—∫ 3: 1 Limit of Quantitation
°√Õßμ—«Õ¬à“ߢÕß “√≈–≈“¬∑’Ë®–∑”°“√ (LOQ) §◊Õ§«“¡‡¢â¡¢âπ∑’ËμË”∑’Ë ÿ¥∑’Ë«‘∏’«‘‡§√“–Àå

«‘‡§√“–Àå¥â«¬ nylon filter membrane ¢π“¥ 0.45 “¡“√∂μ√«®«—¥‰¥âÕ¬à“ß∂Ÿ°μâÕß ∑”‰¥â‚¥¬‡®◊Õ®“ß
‰¡§√Õπ °àÕπ∑”°“√«‘‡§√“–Àå¥â«¬‡§√◊ËÕß HPLC §à“ “√¡“μ√∞“π ®π‰¥â§à“ signal/noise ratio ‡∑à“°—∫

§«“¡‡¢â¡¢âπ¢Õß “√∑’ˉ¥â®“°‡§√◊ËÕß π”¡“§”π«≥§à“ 10 : 1
% recovery
º≈°“√»÷°…“
2.2 Precision ‡ªìπ°“√μ√«® Õ∫§«“¡

‡∑’ˬßμ√ߢÕß«‘∏’«‘‡§√“–Àå ‡æ◊ËÕ¥Ÿ«à“º≈°“√«‘‡§√“–Àå∑’Ë 1. °“√‡μ√’¬¡ “√¡“μ√∞“π “√ D °—∫ “√ 1,
∑”´È”„π·μà≈–§√—Èß ‚¥¬°“√©’¥ “√¡“μ√∞“π·μà≈– 2 ·≈– 3

™π‘¥∑’˧«“¡‡¢â¡¢âπ√–¥—∫°≈“ߢÕß linearity ®”π«π “√¡“μ√∞“π∑—Èß 4 ™π‘¥ ‰¥â·°à “√ D °—∫ “√
6 §√—ÈßμàÕ‡π◊ËÕß°—π ‡√’¬°«à“ intra-day precision 1, 2 ·≈– 3 ‰¥â®“°«‘∏’‡§¡’ —߇§√“–Àå ‚¥¬‰¥âº≈º≈‘μ
·≈–π” “√¡“μ√∞“π‡¥‘¡©’¥´È”Õ’° 6 §√—Èß„π«—πμàÕ¡“ ¢Õß “√·μà≈–™π‘¥¥—ßπ’È “√ D ‰¥âº≈º≈‘μ‡∑à“°—∫

π”§à“∑—Èß Õß«—π¡“§”π«≥ ‡√’¬°«à“ inter-day pre- √âÕ¬≈– 28.27 ‚¥¬ºà“π°“√ —߇§√“–Àå 4 ¢—ÈπμÕπ “√
cision §à“ precision ¢Õß«‘∏’«‘‡§√“–Àå¡—°§”π«≥ 1, 2 ·≈– 3 ‡°‘¥®“°ªØ‘°‘√‘¬“ Steglich reaction

√Ÿª∑’Ë 4 HPLC ‚§√¡“‚μ·°√¡¢Õß “√¡“μ√∞“π “√ D °—∫ “√ 1(A1), 2(A2) ·≈– 3(A3)