Page 49 - วารสารกรมการแพทย์แผนไทยฯ ปีที่ 11 ฉบับที่ 3

258 «“√ “√°“√·æ∑¬å·ºπ‰∑¬·≈–°“√·æ∑¬å∑“߇≈◊Õ° ªï∑’Ë 11 ©∫—∫∑’Ë 3°—𬓬π-∏—𫓧¡ 2556

25
μ“√“ß∑’Ë 1 ¢âÕ¡Ÿ≈ H-NMR (500 MHz) ¢Õß 1 ‡ª√’¬∫‡∑’¬∫°—∫ 3-(3,4-Dihydroxyphenyl) lactic Acid
1
δ (ppm) „π d -acetone ¢Õß
H 6
μ”·Àπàß‚ª√μÕπ δ (ppm) „π d -DMSO ¢Õß 1 3-(3,4-dihydroxy-phenyl)
6
H
25
lactic acid
H-2 6.67 (d, J=11.0 Hz, 1H) 6.79 (d, J=2 Hz, 1H)
OH-3 3.20 (s, 1H) -
OH-4 3.28 (s, 1H) -
H-5 6.57 (d, J= 8.5 Hz, 1H) 6.72 (d, J= 8 Hz, 1H)
H-6 6.68 (dd, J=10.1 Hz, 1H) 6.60 (dd, J=2, 8 Hz, 1H)
H-7 2.61 (dd, J= 9.7, 5.2 Hz, 1H) 2.73 (dd, J= 14, 7 Hz, 1H)
2.81 (dd, J= 9.7, 4.9 Hz, 1H) 2.98 (dd, J= 14, 5 Hz, 1H)
H-8 4.06 (dd, J= 5.2, 4.9 Hz, 1H) 4.32 (dd, J= 5, 7 Hz, 1H)
OH-8 3.21 (s, 1H) -
OH-9 11.0 (s, 1H) -

13
μ“√“ß∑’Ë 2 ¢âÕ¡Ÿ≈ C-NMR (125 MHz) ¢Õß 1 ‡ª√’¬∫‡∑’¬∫°—∫ 3-(3,4-Dihydroxyphenyl) lactic Acid 25
δ (ppm) „π d -acetone ¢Õß
6
C
μ”·Àπàߧ“√å∫Õπ δ (ppm) „π d -DMSO ¢Õß 1 3-(3,4-dihydroxyphenyl)
C 6
25
lactic acid
C-1 132.34 130.2
C-2 117.63 116.2
C-3 145.17 144.9
C-4 143.89 145.9
C-5 118.66 117.7
C-6 123.30 121.9
C-7 41.00 41.0
C-8 74.77 73.0
C-9 181.94 177.2

‡∑à“°—∫ 4.1 π“∑’ ∑’˧«“¡¬“«§≈◊Ëπ 279 π“‚π‡¡μ√ ·Àâߥ⫬§«“¡‡¬Áπ (freeze-dried) ®”π«π 9 μ—«Õ¬à“ß
·≈– “√ 2, 3 ¡’§à“ retention time ‡∑à“°—∫ 28.0, æ∫«à“ ¡’§à“‡©≈’ˬ ± §à“‡∫’ˬ߇∫π¡“μ√∞“π (Mean ±
13.8 π“∑’ ∑’˧«“¡¬“«§≈◊Ëπ 324 π“‚π‡¡μ√ μ“¡≈”¥—∫ SD) §‘¥‡ªìπ√âÕ¬≈– 1.86 ± 0.61, 0.43 ± 0.27, 0.07

‡¡◊ËÕ»÷°…“À“ª√‘¡“≥ “√ 1-3 „π “√ °—¥πÈ”™π‘¥ ± 0.05 ‚¥¬πÈ”Àπ—° μ“¡≈”¥—∫ (√Ÿª∑’Ë 2, μ“√“ß∑’Ë 7)
À¬“∫®“°·¡ß≈—°§“∑’ˇμ√’¬¡¥â«¬«‘∏’°“√√–‡À‘¥πÈ”„Àâ ”À√—∫º≈°“√∑¥ Õ∫ƒ∑∏‘Ï∑“ß™’«¿“æ„π