Page 49 - วารสารกรมการแพทย์แผนไทยฯ ปีที่ 11 ฉบับที่ 3
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258  «“√ “√°“√·æ∑¬å·ºπ‰∑¬·≈–°“√·æ∑¬å∑“߇≈◊Õ°         ªï∑’Ë 11 ©∫—∫∑’Ë 3°—𬓬π-∏—𫓧¡ 2556



                                                                                       25
             μ“√“ß∑’Ë 1 ¢âÕ¡Ÿ≈  H-NMR (500 MHz) ¢Õß 1 ‡ª√’¬∫‡∑’¬∫°—∫ 3-(3,4-Dihydroxyphenyl) lactic Acid
                           1
                                                                        δ  (ppm) „π d -acetone ¢Õß
                                                                         H        6
               μ”·Àπàß‚ª√μÕπ             δ  (ppm) „π d -DMSO ¢Õß 1       3-(3,4-dihydroxy-phenyl)
                                                    6
                                          H
                                                                                      25
                                                                              lactic acid
                    H-2                    6.67 (d, J=11.0 Hz, 1H)        6.79 (d, J=2 Hz, 1H)
                   OH-3                        3.20 (s, 1H)                       -
                   OH-4                        3.28 (s, 1H)                       -
                    H-5                    6.57 (d, J= 8.5 Hz, 1H)        6.72 (d, J= 8 Hz, 1H)
                    H-6                   6.68 (dd, J=10.1 Hz, 1H)       6.60 (dd, J=2, 8 Hz, 1H)
                    H-7                  2.61 (dd, J= 9.7, 5.2 Hz, 1H)  2.73 (dd, J= 14, 7 Hz, 1H)
                                         2.81 (dd, J= 9.7, 4.9 Hz, 1H)  2.98 (dd, J= 14, 5 Hz, 1H)
                    H-8                  4.06 (dd, J= 5.2, 4.9 Hz, 1H)   4.32 (dd, J= 5, 7 Hz, 1H)
                   OH-8                        3.21 (s, 1H)                       -
                   OH-9                        11.0 (s, 1H)                       -




                           13
             μ“√“ß∑’Ë 2 ¢âÕ¡Ÿ≈  C-NMR (125 MHz) ¢Õß 1 ‡ª√’¬∫‡∑’¬∫°—∫ 3-(3,4-Dihydroxyphenyl) lactic Acid 25
                                                                        δ  (ppm) „π d -acetone ¢Õß
                                                                                  6
                                                                         C
               μ”·Àπàߧ“√å∫Õπ            δ  (ppm) „π d -DMSO ¢Õß 1       3-(3,4-dihydroxyphenyl)
                                          C         6
                                                                                      25
                                                                              lactic acid
                    C-1                          132.34                         130.2
                    C-2                          117.63                         116.2
                    C-3                          145.17                         144.9
                    C-4                          143.89                         145.9
                    C-5                          118.66                         117.7
                    C-6                          123.30                         121.9
                    C-7                           41.00                          41.0
                    C-8                           74.77                          73.0
                    C-9                          181.94                         177.2



             ‡∑à“°—∫ 4.1 π“∑’ ∑’˧«“¡¬“«§≈◊Ëπ 279 π“‚π‡¡μ√  ·Àâߥ⫬§«“¡‡¬Áπ (freeze-dried) ®”π«π 9 μ—«Õ¬à“ß
             ·≈– “√ 2, 3 ¡’§à“ retention time ‡∑à“°—∫ 28.0,  æ∫«à“ ¡’§à“‡©≈’ˬ ± §à“‡∫’ˬ߇∫π¡“μ√∞“π (Mean ±
             13.8 π“∑’ ∑’˧«“¡¬“«§≈◊Ëπ 324 π“‚π‡¡μ√ μ“¡≈”¥—∫  SD) §‘¥‡ªìπ√âÕ¬≈– 1.86 ± 0.61, 0.43 ± 0.27, 0.07

             ‡¡◊ËÕ»÷°…“À“ª√‘¡“≥ “√ 1-3 „π “√ °—¥πÈ”™π‘¥  ± 0.05 ‚¥¬πÈ”Àπ—° μ“¡≈”¥—∫ (√Ÿª∑’Ë 2, μ“√“ß∑’Ë 7)
             À¬“∫®“°·¡ß≈—°§“∑’ˇμ√’¬¡¥â«¬«‘∏’°“√√–‡À‘¥πÈ”„Àâ   ”À√—∫º≈°“√∑¥ Õ∫ƒ∑∏‘Ï∑“ß™’«¿“æ„π
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