Page 48 - วารสารกรมการแพทย์แผนไทยฯ ปีที่ 11 ฉบับที่ 3
P. 48

Journal of Thai Traditional & Alternative Medicine  Vol. 11 No. 3 September-December 2013 257



               albicans) ¥â«¬«‘∏’ MTT Assay ‚¥¬§«“¡‡¢â¡¢âπ  trum ¢Õß “√ 1-3 · ¥ß«à“  “√∑—Èß “¡™π‘¥ ‰¥â·°à
                Ÿß ÿ¥¢Õßμ—«Õ¬à“ß §◊Õ 25 ‰¡‚§√°√—¡/¡‘≈≈‘≈‘μ√ ·≈–  3-(3,4-Dihydroxy- phenyl)lactic acid (1):

               IC  ¢Õß positive control §◊Õ amphotericin B  [C H O ] FAB-MS m/z (% relative intensity):
                50                                         9 10 5
                                                                  +
               ‡∑à“°—∫ 0.049 ‰¡‚§√°√—¡/¡‘≈≈‘≈‘μ√ ∑—Èßπ’È §«“¡‡¢â¡  197 (M-1)  (100), 111 (10). Rosmarinic acid
               ¢âπ¢Õß “√≈–≈“¬μ—«Õ¬à“ß∑—ÈßÀ¡¥∑’ˇ≈◊Õ°„™âμâÕ߉¡à  (2): [C H O ] TOF-MS (ES+) m/z (% relative
                                                              18 15 8
                                                                              +
               ‡ªìπæ‘…μàÕ‡´≈≈å∑’Ë„™â∑¥ Õ∫                intensity): 383.0 (M+Na)  (100), 360.2 (M-Na) +
                                                         (10), 185.1 (15), 163.0 (42.5). Caffeic acid [3-
                            º≈°“√»÷°…“
                                                         (3,4-dihydroxyphenyl)-propenoic acid] (3):

                   ®“°º≈°“√·¬° “√ °—¥πÈ”™π‘¥À¬“∫®“° à«π  [C H O ] TOF-MS (ES+) m/z (% relative in-
                                                           8 4 5
                                                                              +
               ‡Àπ◊Õ¥‘π·¡ß≈—°§“¥â«¬°“√ partition ·≈–„™â‡∑§π‘§  tensity): 203.03 (M+Na)  (15), 181.05 (M+H-
                                                            +
                                                                                +
               CC æ∫«à“·¬° “√∫√‘ ÿ∑∏‘ω¥â 3 ™π‘¥ ‚¥¬ “√ 1 ¡’  Na)  (15), 163.03 (M-H O)  (100), 150.12 (10)
                                                                             2
               ≈—°…≥–‡ªìπº≈÷°§≈⓬ª√‘´÷¡ ¥Ÿ¥§«“¡™◊Èπßà“¬ ‰¡à¡’ ’  ®“°º≈°“√·¬° “√æ∫«à“ ‰¥â “√ 1, 2 ·≈– 3 ®”π«π
                “√ 2 ·≈– 3 ¡’≈—°…≥–‡ªìπºß amorphous  ’¢“«  180, 120 ·≈– 120 ¡‘≈≈‘°√—¡ μ“¡≈”¥—∫ §‘¥‡ªìπ
               ‡¡◊ËÕæ‘ Ÿ®πå Ÿμ√‚§√ß √â“ß∑“߇§¡’¥â«¬‡∑§π‘§ spec-  √âÕ¬≈–¢Õߺ≈º≈‘μ (%yield) ‡∑à“°—∫ 0.81, 0.033 ·≈–
                            1
               troscopy æ∫«à“  H-NMR spectra ¡’§à“ chemi-  0.013 ‚¥¬πÈ”Àπ—° μ“¡≈”¥—∫ ‡¡◊ËÕ‡ª√’¬∫‡∑’¬∫°—∫
               cal shift (δ, ppm), splitting pattern ·≈– cou-   “√ °—¥πÈ”™π‘¥À¬“∫®“°·¡ß≈—°§“
                                        13
               pling constant (J, Hz) √«¡∑—Èß  C-NMR spec-    ‡¡◊ËÕ«‘‡§√“–Àå “√ °—¥πÈ”™π‘¥À¬“∫®“°·¡ß≈—°
               tra  Õ¥§≈âÕß°—∫º≈°“√»÷°…“„π√“¬ß“π°“√«‘®—¬∑’Ë  §“¥â«¬«‘∏’‚§√¡“‚∑°√“øï ¡√√∂π– Ÿß ‚¥¬„™â “√ 1-
                       25-27
               μ’æ‘¡æå·≈â«   (μ“√“ß∑’Ë 1-6) ·≈–®“°º≈ MS spec-  3 ‡ªìπ “√‡∑’¬∫ æ∫«à“ “√ 1 ¡’§à“ retention time


























               √Ÿª∑’Ë 1  Ÿμ√‚§√ß √â“ß∑“߇§¡’¢Õß 3-(3,4-dihydroxyphenyl) lactic acid (1), rosmarinic acid (2) ·≈– caffeic
                     acid (3)
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