Page 48 - วารสารกรมการแพทย์แผนไทยฯ ปีที่ 11 ฉบับที่ 3

Journal of Thai Traditional & Alternative Medicine Vol. 11 No. 3 September-December 2013 257

albicans) ¥â«¬«‘∏’ MTT Assay ‚¥¬§«“¡‡¢â¡¢âπ trum ¢Õß “√ 1-3 · ¥ß«à“ “√∑—Èß “¡™π‘¥ ‰¥â·°à
Ÿß ÿ¥¢Õßμ—«Õ¬à“ß §◊Õ 25 ‰¡‚§√°√—¡/¡‘≈≈‘≈‘μ√ ·≈– 3-(3,4-Dihydroxy- phenyl)lactic acid (1):

IC ¢Õß positive control §◊Õ amphotericin B [C H O ] FAB-MS m/z (% relative intensity):
50 9 10 5
+
‡∑à“°—∫ 0.049 ‰¡‚§√°√—¡/¡‘≈≈‘≈‘μ√ ∑—Èßπ’È §«“¡‡¢â¡ 197 (M-1) (100), 111 (10). Rosmarinic acid
¢âπ¢Õß “√≈–≈“¬μ—«Õ¬à“ß∑—ÈßÀ¡¥∑’ˇ≈◊Õ°„™âμâÕ߉¡à (2): [C H O ] TOF-MS (ES+) m/z (% relative
18 15 8
+
‡ªìπæ‘…μàÕ‡´≈≈å∑’Ë„™â∑¥ Õ∫ intensity): 383.0 (M+Na) (100), 360.2 (M-Na) +
(10), 185.1 (15), 163.0 (42.5). Caffeic acid [3-
º≈°“√»÷°…“
(3,4-dihydroxyphenyl)-propenoic acid] (3):

®“°º≈°“√·¬° “√ °—¥πÈ”™π‘¥À¬“∫®“° à«π [C H O ] TOF-MS (ES+) m/z (% relative in-
8 4 5
+
‡Àπ◊Õ¥‘π·¡ß≈—°§“¥â«¬°“√ partition ·≈–„™â‡∑§π‘§ tensity): 203.03 (M+Na) (15), 181.05 (M+H-
+
+
CC æ∫«à“·¬° “√∫√‘ ÿ∑∏‘ω¥â 3 ™π‘¥ ‚¥¬ “√ 1 ¡’ Na) (15), 163.03 (M-H O) (100), 150.12 (10)
2
≈—°…≥–‡ªìπº≈÷°§≈⓬ª√‘´÷¡ ¥Ÿ¥§«“¡™◊Èπßà“¬ ‰¡à¡’ ’ ®“°º≈°“√·¬° “√æ∫«à“ ‰¥â “√ 1, 2 ·≈– 3 ®”π«π
“√ 2 ·≈– 3 ¡’≈—°…≥–‡ªìπºß amorphous ’¢“« 180, 120 ·≈– 120 ¡‘≈≈‘°√—¡ μ“¡≈”¥—∫ §‘¥‡ªìπ
‡¡◊ËÕæ‘ Ÿ®πå Ÿμ√‚§√ß √â“ß∑“߇§¡’¥â«¬‡∑§π‘§ spec- √âÕ¬≈–¢Õߺ≈º≈‘μ (%yield) ‡∑à“°—∫ 0.81, 0.033 ·≈–
1
troscopy æ∫«à“ H-NMR spectra ¡’§à“ chemi- 0.013 ‚¥¬πÈ”Àπ—° μ“¡≈”¥—∫ ‡¡◊ËÕ‡ª√’¬∫‡∑’¬∫°—∫
cal shift (δ, ppm), splitting pattern ·≈– cou- “√ °—¥πÈ”™π‘¥À¬“∫®“°·¡ß≈—°§“
13
pling constant (J, Hz) √«¡∑—Èß C-NMR spec- ‡¡◊ËÕ«‘‡§√“–Àå “√ °—¥πÈ”™π‘¥À¬“∫®“°·¡ß≈—°
tra Õ¥§≈âÕß°—∫º≈°“√»÷°…“„π√“¬ß“π°“√«‘®—¬∑’Ë §“¥â«¬«‘∏’‚§√¡“‚∑°√“øï ¡√√∂π– Ÿß ‚¥¬„™â “√ 1-
25-27
μ’æ‘¡æå·≈â« (μ“√“ß∑’Ë 1-6) ·≈–®“°º≈ MS spec- 3 ‡ªìπ “√‡∑’¬∫ æ∫«à“ “√ 1 ¡’§à“ retention time

√Ÿª∑’Ë 1 Ÿμ√‚§√ß √â“ß∑“߇§¡’¢Õß 3-(3,4-dihydroxyphenyl) lactic acid (1), rosmarinic acid (2) ·≈– caffeic
acid (3)