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Journal of Thai Traditional & Alternative Medicine Vol. 7 No. 2-3 May-December 2009 Òıı

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Abstract
Toxicity Study of Lupinifolin from Stem of Derris reticulata Craib
Songpol Chivapat* , Pranee Chavalittumrong , Aimmanas Attawish*,
†
,‡
Noppamas Soonthornchareonnon #
*Medicinal Plant Research Institute, Senior Technical Office, Department of Medical Sciences
†
‡ Department of Pharmacognosy, Faculty of Pharmacy, Mahidol University, Corresponding author
#
Lupinifolin is a flavonone compound expressing high efficacy against Herpes simplex virus 1 (HSV-1).
The objective of this study was to investigate the toxicity of lupinifolin from the stem of Derris reticulata Craib.
Oral administration of lupinifolin in mice at the dose of 5 mg/kg, which was equivalent to an 8,300-fold thera-
peutic dose, showed no acute toxicity. A subacute toxicity study was performed on six groups of Wistar rats
for 28 days. Groups 1 and 2 were control groups given distilled water and 1 percent tragacanth, respectively.
Groups 3 to 6 were administered lupinifolin orally at doses of 0.6, 6.0, 3.0 and 60 mg/kg/day, which were
equivalent to 1, 10, 50 and 100 times the therapeutic dose, respectively. The results revealed that lupinifolin
did not affect body weight, food consumption or the animals’ health. Female rats receiving lupinifolin at the
dose of 60 mg/kg/day had a significant increase in WBC and a significant decrease in neutrophils when com-
pared with the water control group, but these changes were within normal ranges. Male rats receiving lupinifolin
at doses of 30 and 60 mg/kg/day and female rats receiving lupinifolin at 60 mg/kg/day had a significantly
higher sodium level than the water control groups; nevertheless, the increase was within the normal range.
The potassium level in female rats receiving lupinifolin at doses of 30 and 60 mg/kg/day was significantly lower
than that of the water control group. Histopathological results of some visceral organs in the lupinifolin-
treated groups showed no dose-dependent alterations; therefore, lupinifolin from D. reticulata should be con-
sidered safe for the treatment of HSV1-infected wounds.
Key words: Lupinifolin, Derris reticulata, toxicity