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ÿ¿‘ √“ ·®âß «à“ß , ¡∫—μ‘ ‰μ√»√’»‘≈ªá , ՗ߧ≥“ Õ‘πμ“ , ÿ«æ√ ‡À≈◊Õߢ¡‘Èπ , «’√– «ß»å§”
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¿“§«‘™“™’««‘∑¬“ §≥–«‘∑¬“»“ μ√å ¡À“«‘∑¬“≈—¬‡™’¬ß„À¡à
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∂“∫—πæ—≤π“ªí≠≠“‰∑ ®—ßÀ«—¥‡™’¬ß„À¡à
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¿“§«‘™“‡§¡’ §≥–«‘∑¬“»“ μ√å ¡À“«‘∑¬“≈—¬‡™’¬ß„À¡à

À≈—°°“√·≈–‡Àμÿº≈ ‚√§μàÕ¡≈Ÿ°À¡“°‚μ (benign prostatic hypertrophy; BPH) ‡ªìπªí≠À“ ÿ¢¿“æ„π‡æ»
™“¬∑’Ë¡’Õ“¬ÿμ—Èß·μà 50 ªï¢÷Èπ‰ª „πª√–‡∑»‰∑¬æ∫ ∂‘μ‘¢Õß‚√§μàÕ¡≈Ÿ°À¡“°‚μ¡’·π«‚π⡇æ‘Ë¡ Ÿß¢÷Èπ¡“° ‚√§μàÕ¡≈Ÿ°
À¡“°‚μ¡’ “‡Àμÿ®“°§«“¡º‘¥ª°μ‘„π°“√‡æ‘Ë¡®”π«π‡´≈≈å¢Õß°“√·∫àßμ—«„πμàÕ¡≈Ÿ°À¡“°®π∑”„Àâ¢π“¥¢ÕßμàÕ¡≈Ÿ°
À¡“°„À≠à¢÷Èπ°«à“ª°μ‘ ´÷Ëß°“√‡ªìπμàÕ¡≈Ÿ°À¡“°‚μ¡’‚Õ°“ æ—≤𓇪ìπ¡–‡√ÁßμàÕ¡≈Ÿ°À¡“°‰¥â ‚¥¬æ∫«à“¡’μ”√—∫ ¡ÿπ‰æ√
æ◊Èπ∫â“π¢Õß¿“§‡Àπ◊Õ„π√Ÿª·∫∫¬“μâ¡∑’Ë„™â√—°…“‚√§μàÕ¡≈Ÿ°À¡“°‚μ ªí≠À“°Á§◊Õμ”√—∫ ¡ÿπ‰æ√¥—ß°≈à“«π’È à«π„À≠à„™â‰¥âº≈
·μàÀ≈“¬Ê°√≥’°≈—∫‰¡à‰¥âº≈

«—μ∂ÿª√– ß§å ‡æ◊ËÕ∑¥ Õ∫ƒ∑∏‘χ∫◊ÈÕß·√°„π°“√μàÕμâ“πμàÕ¡≈Ÿ°À¡“°‚μ„π‡´≈≈凙◊ÈÕ “¬μàÕ¡≈Ÿ°À¡“°‚μ·≈–‡´≈≈å
¡–‡√ÁßμàÕ¡≈Ÿ°À¡“°¢Õß “√ °—¥ ¡ÿπ‰æ√®“°μ”√—∫¬“√—°…“‚√§μàÕ¡≈Ÿ°À¡“°‚μ¢Õ߉∑¬
«‘∏’¥”‡π‘π°“√ π” “√ °—¥æ◊™ ¡ÿπ‰æ√®“°μ”√—∫¬“√—°…“‚√§μàÕ¡≈Ÿ°À¡“°‚μ¢Õ߉∑¬ π¡«—« (Anomianthus dulcis
(Dunal) J. Sinclair), π¡§«“¬ (Uvaria hahinii Sincl.) ·≈–Ω“ß (Caesalpinia sappan Linn.) ∑’Ë °—¥¥â«¬μ—«∑”
≈–≈“¬ 3 ™π‘¥ §◊Õ dichloromethane, 95% ethanol ·≈–πÈ”°≈—Ëπ ‡æ◊ËÕ„À≥⠓√ °—¥∑—ÈßÀ¡¥ (crude) ¡“∑¥ Õ∫ƒ∑∏‘Ï
μàÕμâ“πμàÕ¡≈Ÿ°À¡“°‚μ„π‡´≈≈凙◊ÈÕ “¬μàÕ¡≈Ÿ°À¡“°‚μ·≈–‡´≈≈凙◊ÈÕ “¬¡–‡√ÁßμàÕ¡≈Ÿ°À¡“° ‚¥¬°√–∫«π°“√μ√«®
Õ∫∑’Ë„™â„πß“π«‘®—¬π’ȧ◊Õ μ√«® Õ∫°“√ÕÕ°ƒ∑∏‘Ï·≈–°≈‰°‡∫◊ÈÕßμâπ„π°“√μàÕμâ“πμàÕ¡≈Ÿ°À¡“°‚μ„π√–¥—∫‡´≈≈å ª√–°Õ∫¥â«¬
°“√∑¥ Õ∫§«“¡‡ªìπæ‘…√–¥—∫‡´≈≈å (cytotoxic assay), °“√μàÕμâ“π°“√‡æ‘Ë¡®”π«π‡´≈≈å (anti-proliferation assay)
·≈–°“√¬—∫¬—Èß°“√ √â“ß°≈ÿࡇ´≈≈å (clonogenic assay)
º≈°“√»÷°…“ æ∫«à“ “√ °—¥∑—ÈßÀ¡¥ (crude) ¢Õß “√ °—¥ ¡ÿπ‰æ√∑—Èß 10 “√‰¡à· ¥ß§«“¡‡ªìπæ‘…μàÕ‡´≈≈å∑—Èß
2 ™π‘¥ ‡¡◊ËÕ‡´≈≈剥â√—∫ “√ °—¥‡ªìπ‡«≈“ 24 ™—Ë«‚¡ß °“√»÷°…“°“√μàÕμâ“π°“√‡æ‘Ë¡®”π«π‡´≈≈å¢Õß “√ °—¥„π‡´≈≈å DU145
æ∫«à“¡’ “√∑’ˉ¡à· ¥ß§«“¡ “¡“√∂„π°“√ÕÕ°ƒ∑∏‘ÏμàÕμâ“π°“√‡æ‘Ë¡®”π«π‡´≈≈å §◊Õ Ω“ß∑’Ë °—¥¥â«¬ dichloromethane
·≈–π¡«—« Ω“ß ·≈– “√ °—¥√«¡∑—Èß “¡™π‘¥ (mix) ∑’Ë °—¥¥â«¬°“√μâ¡ ‚¥¬¡’§à“ IC ¡“°°«à“ 1,000 μg/ml „π‡«≈“ 72
50
™—Ë«‚¡ß à«π„π‡´≈≈å LNCaP æ∫«à“ “√∑’Ë· ¥ß·π«‚πâ¡„π°“√μàÕμâ“πÀ√◊Õ¬—∫¬—Èß°“√‡æ‘Ë¡®”π«π‡´≈≈剥ⷰà π¡§«“¬
2
1
·≈–Ω“ß∑’Ë °—¥¥â«¬ dichloromethane ·≈–Ω“ß∑’Ë °—¥¥â«¬ ethanol ´÷Ëß¡’§à“ IC ‡∑à“°—∫ 3.34±10 , 5.11±10 ·≈–
50
2
3.20±10 μg/ml μ“¡≈”¥—∫ °“√∑¥ Õ∫ƒ∑∏‘Ï„π°“√¬—∫¬—Èß°“√ √â“ß°≈ÿࡇ´≈≈åÀ√◊Õ‚§‚≈π’¢Õ߇´≈≈å æ∫«à“ “√ °—¥∑ÿ°μ—«
· ¥ß°“√¬—∫¬—Èß°“√ √â“ß°≈ÿࡇ´≈≈å„π‡´≈≈å∑—Èß Õß™π‘¥ ‚¥¬¢÷ÈπÕ¬Ÿà°—∫§«“¡‡¢â¡¢âπ¢Õß “√ °—¥∑’ˉ¥â√—∫ (dose-dependent)
¢âÕ √ÿª ®“°°“√»÷°…“‡∫◊ÈÕßμâππ’È· ¥ß„Àâ‡ÀÁπ«à“ “√ °—¥ ¡ÿπ‰æ√∑—ÈßÀ¡¥ (crude) ¡’ “√ÕÕ°ƒ∑∏‘ÏÀ≈“¬Õ¬à“ߪ–ªπ
°—π ∑—Èß “√∑’ËÕÕ°ƒ∑∏‘Ï°√–μÿâπ‡´≈≈å·≈– “√∑’ËÕÕ°ƒ∑∏‘ÏμàÕμâ“π°“√‡æ‘Ë¡®”π«π‡´≈≈åμàÕ‡´≈≈å¡–‡√ÁßμàÕ¡≈Ÿ°À¡“° ‚¥¬¡’°“√
ÕÕ°ƒ∑∏‘Ï·∫∫¢÷Èπ°—∫§«“¡‡¢â¡¢âπ¢Õß “√ °—¥∑’ˉ¥â√—∫ (dose-dependent manner) °“√«‘®—¬„π≈”¥—∫μàÕ‰ª®–‡ªìπ°“√
μ√«® Õ∫°≈‰°‡∫◊ÈÕßμâπ¢Õß “√∑’ËÕÕ°ƒ∑∏‘Ï„π√–¥—∫‡´≈≈å